Abstract
Possibilities and limitations in the use of in vivo cytogenetic tests on somatic cells are discussed. Special attention is focused on the problems raised by the relative insensivity of the available procedures. It is emphasized that in planning screening tests the accumulated experience from studies with known chromosome breaking chemicals must be taken into account; theoretical considerations should not override the actual observations. In the Appendix, technical details of the micronucleus test are given. Furthermore, some thoughts are expressed concerning cytogenetic testing on germ cells and embryos.
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D. T. Arakaki andW. Schmid,chemical Mutagenesis. The Chinese Hamster Bone Marrow as an in vivo Test System. II. Correlation with in vitro Results on Chinese Hamster Fibroblasts and Human Fibroblasts and Lymphocytes, Humangenetik11, 119–131 (1971).
K. Boller andW. Schmid,Chemische Mutagenese beim Säuger. Das Knochenmark des Chinesischen Hamsters als In-vivo-Testsystem. Hämatologische Befunde nach Behandlung mit Trenimon, Humangenetik11, 35–54 (1970).
Chromosome Methodologies in Mutation Testing. Report of the Ad Hoc Committee of the Environmental Mutagen Society and The Institute for Medical Research, Toxic. appl. Pharmac.22, 269–275 (1972).
P. K. Datta, H. Frigger andE. Schleiermacher,The Effect of Chemical Mutagens on the Mitotic Chromosomes of the Mouse, in vivo (Eds. F. Vogel and G. Röhrborn; Springer-Verlag, Berlin-Heidelberg-New York 1970).
M. von Ledebur andW. Schmid,The Micronucleus Test. Methodological Aspects Mutation Res., in press.
B. Matter andW. Schmid,Trenimon-Induced Chromosomal Damage in Bone Marrow Cells of Six Mammalian Species, Evaluated by the Micronucleus Test, Mutation Res.12, 417–425 (1971).
W. Schmid andG. R. Staiger,Chromosome Studies on Bone Marrow from Chinese Hamsters Treated with Benzodiazepine Tranquilizers and Cyclophosphamide, Mutation Res.7, 99–108 (1969).
W. Schmid, D. T. Arakaki, N. A. Breslau andJ.C. Culbertson,Chemical Mutagenesis. The Chinese Hamster Bone Marrow as an in vivo Test System. I. Cytogenetic Results on Basic Aspects of the Methodology, Obtained with Alkylating Agents, Humangenetik11, 103–118 (1971).
J. Schubert,A Program to Abolish, Harmful Chemicals, Ambio3, 79–89 (1972).
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Lecture held at the International Workshop on Mutagenicity Testing of Drugs and Other Chemicals, Zürich, 2–5 October 1972.
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Schmid, W. Chemical mutagen testing on in vivo somatic mammalian cells. Agents and Actions 3, 77–85 (1973). https://doi.org/10.1007/BF01986538
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DOI: https://doi.org/10.1007/BF01986538